IMM & SLS & LBM & IDG McGovern Institute for Brain Research & BIOPIC & CLS

Research Seminar

Title：Pushing the Envelope in Super-resolution Live Imaging

Speaker：Dong Li, Ph.D.

Janelia Research Campus, Howard Hughes Medical Institute, USA

Time：2015年5月16日（星期六）上午10:00-12:00

Place：北京大学英杰交流中心306会议室

Host：北京大学分子医学研究所 程和平 (Tel. 6276-5957)

Every superresolution technique provides the theoretical capability to resolve ultrastructure beyond the diffraction barrier, but many of them encounter practical limitations when imaging nano-scale dynamics in living biological samples, especially over long-term course and large field of view. Structured illumination microscopy (SIM) stands out in the context of live imaging, since many fewer raw images and much lower light levels are required. In addition, its higher photon efficiency and better high frequency support in its optical transfer function (OTF) require less photon emission from specimen than other techniques to achieve a given resolution. Its well-known disadvantage is that its resolution is usually limited to ~100 nm. In this representation, I will report two approaches to extend the resolution into sub-100 nm regime, while retaining the above merits to accomplish sub-second imaging speed over scores of time points, without apparent phototoxicity. These methods permit us to observe dynamics unresolvable by conventional means, such as paired myosin head domains binding with actin filaments, the assembly as dissolution of ring-like clathrin and caveolin vesicles, as well as mitochondrial fission, fusion and migration, etc.

欢迎各位老师同学积极参加！