Title: Site-specific induction of oxidative DNA damage reveals differences in recruitment of repair proteins to heterochromatin and euchromatin

Speaker：Li Lan

Assistant Professor,UPCI, Department of Microbiology and Molecular Genetics,

University of Pittsburgh School of Medicine ，Pittsburgh, PA

Time:2014年12月08日(星期一)14:15-15:15

Place:生命科学学院411会议室

Host: 徐冬一（电话：62759816）

abstract

Oxidative DNA damage caused by reactive oxygen species (ROS), generated by environmental toxicants (e.g., chemicals or irradiation) or during cellular metabolism, is responsible for genomic instability, epigenetic information changes, leads to tumorigenesis and aging. To understand the oxidative damage response in situ, we have developed several unique methods to induce ROS induced DNA damage at specific genome loci in live cells. These methods together with biochemical and biology assays allow us to 1) directly induce oxidative DNA damage at specific genome loci or at genome site and telomeres, 2) to visualize the DNA damage response at distinct genome loci in real time with 3D resolution in a single cell nucleus, 3) to understand how chromatin remodeling, histone epigenetic alteration and transcription are involved in DNA damage response and 4) to observe the cellular effects of genome damage versus telomeric damage. I will focus on discussing 1) the mechanisms of how cells protect affects genome stability against DNA damage via altering epigenetic information; 2) the links of these process in tumorigenesis, aging and neurodevelopmental defects by applying these approaches in current studies.