生命科学学院公共仪器中心学术报告
题目：A genome-wide analysis of protein translocation in quiescent yeast cells
报告人：Jun-Yi Leu, Ph.D.
Research Fellow, Institute of Molecular Biology, Academia Sinica, Taiwan.
时间：2014-4-14（周一），10:00-11:00 AM
地点：北京大学新生物楼208会议室
联系人：生命学院公共仪器中心，李晓晨（电话：62751853）
Modern human societies are facing many problems caused by population aging. A thorough understanding of physiological and genetic bases of the aging process is critical for developing medical treatments to deal with this issue. The budding yeast has been shown to be a useful model system for aging research. In our previous studies, we also observed that many proteins relocalize and form reversible cytoplasmic granules in chronologically aged (quiescent) yeast cells. Mutations in the granule components reduce viability of the aged cells. Protein translocation is an important strategy for cells to regulate protein functions. To understand the complete composition and function of these granules, we perform a systematic genome-wide analysis of protein translocalization in quiescent cells using the yeast GFP collection. We find that about 353 of the GFP-tagged proteins form non-mitochondrial dot-like structures in quiescent cells. By examining colocalization patterns between these proteins and markers of different cytoplasmic granules, we discover many new members in Hsp42 stationary-phase granules, actin bodies, proteasome storage granules, and processing bodies. Some of the granules share overlapping components, suggesting that distinct granules interact with each other. Besides, the granules are dynamic and able to respond to other environmental stresses. Finally, we observe novel reversible cytoplasmic granules which do not colocalize with any known granule.
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