Speaker：Changfeng Chen（陈长风）Liyuan Zhu（朱理源）Zihan Li（李子涵）Zhecheng Huang（黄哲成）

Time：10:00 - 13:00

Location：Room 348, Wangkezhen Building

Abstract

Animal cloning can be achieved by somatic cell nuclear transfer (SCNT) which reprograms the nuclear to a totipotent state. More than 20 mammalian species have been successfully cloned since the birth of Dolly the Sheep in 1997 (Matoba and Zhang, 2018). However, cloning efficiency is extremely low, which implicates significant barriers during nuclear reprogramming and the following development. Over the past few decades, great effort has been made in unraveling mechanisms and potential barriers of SCNT reprogramming. Several kinds of epigenetic factors were demonstrated to be important barriers in this process, including aberrant Xist activation, histone modification, DNA methylation and so on (Matoba and Zhang, 2018). Erasure or restriction of these factors contributed to increase of cloning efficiency (Kishigami et al, 2006; Inoue et al, 2010; Chung et al, 2015). By use of histone demethylase Kdm4d mRNA and histone deacetylase inhibitor trichostatin A (TSA) combined with improved SCNT methods, the first non-human primate species was cloned last year (Liu et al, 2018). Investigating barriers of SCNT reprogramming will also facilitate the potential application of reproductive and therapeutic cloning. 

Guest information:

1. Matoba, Shogo, and Yi Zhang. "Somatic cell nuclear transfer reprogramming: mechanisms and applications." Cell stem cell(2018).

Link: https://doi.org/10.1016/j.stem.2018.06.018

1. Chung, Young Gie, et al. "Histone demethylase expression enhances human somatic cell nuclear transfer efficiency and promotes derivation of pluripotent stem cells." Cell Stem Cell17.6 (2015): 758-766.

DOI: https://doi.org/10.1016/j.stem.2015.10.001

2. Liu, Zhen, et al. "Cloning of macaque monkeys by somatic cell nuclear transfer." Cell 172.4 (2018): 881-887.

Link: https://doi.org/10.1016/j.cell.2018.01.020