Speaker：Weitong Gao（高维通）Can Liu（刘灿）Xingyu Ru（茹星语）Chenxi Sun（孙晨曦）Youran Yang（杨悠然）

Time：10:00 - 13:00

Abstract:

One of the problems which neuron biologists want to solve is to visualize a pathway, or to understand the structure of it. They have tried many methods before, such as using fluorescent protein (or small molecules), optogenetics and cryoetching. All of these have their advantages, along with severe disadvantages. Professor Sergiu P. Pașca combined several technological advances (human embryonic stem (hES) cells culturing, induced pluripotent stem (iPS) cells reprogramming and 3D brain cultures ) together to form specific 3D brain cultures which can model complex cell-cell interactions and generate circuits in human brain assembloids. Further, this assembloid can be tested for clinic and psychological use. During the meeting, professor Pașca showed most recent data from his lab, they tried to combine three kinds of tissue, which induced from hES, and watch the neurogeneration among them. By using fluorescent microscopy, we can capture all the movement after excitation easily. Actually, I think this work is tremendously exciting and if possible, I want to invite professor Pașca to attend our JC next semester.

Guest information:

2. Yaonan Liu (Graduate student)

Recommend Literatures：

1. Pașca, Sergiu P. "The rise of three-dimensional human brain cultures." Nature 553.7689 (2018): 437.

DOI: https://doi.org/10.1038/nature25032

DOI: https://doi.org/10.1038/nmeth.4304

2. Sloan, Steven A., et al. "Human astrocyte maturation captured in 3D cerebral cortical spheroids derived from pluripotent stem cells. "  Neuron 95.4 (2017): 779-790.

DOI: https://doi.org/10.1016/j.neuron.2017.07.035