Speaker：Aoyue Mao(茅傲岳), Yuhao Zhu(朱宇豪), Jiashu Shen(申嘉澍), Yaxuan Zhou(周雅轩) and Zelin Wei(魏泽林)

Abstract:

RNA has irreplaceable and diverse roles in organisms, but molecular tools to manipulate and measure it are limited. With the development of CRISPR/Cas system, scientists begin to dig into its potentiality of targeting RNA, besides its well-known function of DNA-targeting. In this lecture, we will focus on the recently developed RNA-guided RNA-targeting CRISPR-Cas13 system, mainly highlighting on its features, advantages and applications.

Guest:

Zhiwei Huang(黄志伟) from Harbin Institute of Technology

http://homepage.hit.edu.cn/huangzhiwei

Recommend Literatures：

Review:

1. Shmakov S, Abudayyeh OO, Makarova KS, et al. Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems. MOLCEL. 2015;60(3):385-397.

https://www.cell.com/molecular-cell/pdf/S1097-2765(15)00775-3.pdf

Papers:

1. Konermann S, Lotfy P, Brideau NJ, Oki J, Shokhirev MN, Hsu PD. Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors. Cell. March 2018:1-27.

https://www.cell.com/cell/pdf/S0092-8674(18)30207-1.pdf

2. Cox DBT, Gootenberg JS, Abudayyeh OO, et al. RNA editing with CRISPR-Cas13. Science. 2017;358(6366):1019-1027.

http://science.sciencemag.org/content/358/6366/1019/tab-pdf